This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 78.92 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 275,156 (9.1%) Reads that were too short: 24,731 (0.8%) Reads written (passing filters): 250,425 (8.3%) Total basepairs processed: 905,907,359 bp Total written (filtered): 38,166,029 bp (4.2%) === Adapter 1 === Sequence: CACTCT; Type: regular 5'; Length: 6; Trimmed: 275156 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 167 734.8 0 167 7 1747 734.8 0 1747 8 75 734.8 0 75 9 8758 734.8 0 8758 10 48 734.8 0 48 11 14 734.8 0 14 12 11 734.8 0 11 13 11 734.8 0 11 14 13 734.8 0 13 15 13 734.8 0 13 16 10 734.8 0 10 17 26 734.8 0 26 18 47 734.8 0 47 19 25 734.8 0 25 20 8 734.8 0 8 21 19 734.8 0 19 22 16 734.8 0 16 23 11 734.8 0 11 24 21 734.8 0 21 25 14 734.8 0 14 26 19 734.8 0 19 27 11 734.8 0 11 28 11 734.8 0 11 29 9 734.8 0 9 30 18 734.8 0 18 31 17 734.8 0 17 32 14 734.8 0 14 33 12 734.8 0 12 34 16 734.8 0 16 35 36 734.8 0 36 36 14 734.8 0 14 37 13 734.8 0 13 38 60 734.8 0 60 39 13 734.8 0 13 40 12 734.8 0 12 41 29 734.8 0 29 42 36 734.8 0 36 43 18 734.8 0 18 44 51 734.8 0 51 45 24 734.8 0 24 46 133 734.8 0 133 47 390 734.8 0 390 48 50 734.8 0 50 49 14 734.8 0 14 50 13 734.8 0 13 51 17 734.8 0 17 52 14 734.8 0 14 53 25 734.8 0 25 54 29 734.8 0 29 55 37 734.8 0 37 56 20 734.8 0 20 57 35 734.8 0 35 58 13 734.8 0 13 59 13 734.8 0 13 60 6 734.8 0 6 61 18 734.8 0 18 62 25 734.8 0 25 63 17 734.8 0 17 64 12 734.8 0 12 65 10 734.8 0 10 66 14 734.8 0 14 67 14 734.8 0 14 68 14 734.8 0 14 69 17 734.8 0 17 70 24 734.8 0 24 71 35 734.8 0 35 72 71 734.8 0 71 73 15 734.8 0 15 74 21 734.8 0 21 75 8 734.8 0 8 76 16 734.8 0 16 77 14 734.8 0 14 78 17 734.8 0 17 79 15 734.8 0 15 80 35 734.8 0 35 81 19 734.8 0 19 82 41 734.8 0 41 83 39 734.8 0 39 84 21 734.8 0 21 85 23 734.8 0 23 86 19 734.8 0 19 87 21 734.8 0 21 88 50 734.8 0 50 89 63 734.8 0 63 90 51 734.8 0 51 91 81 734.8 0 81 92 112 734.8 0 112 93 48 734.8 0 48 94 190 734.8 0 190 95 189 734.8 0 189 96 107 734.8 0 107 97 63 734.8 0 63 98 46 734.8 0 46 99 50 734.8 0 50 100 34 734.8 0 34 101 37 734.8 0 37 102 48 734.8 0 48 103 36 734.8 0 36 104 193 734.8 0 193 105 230 734.8 0 230 106 275 734.8 0 275 107 109 734.8 0 109 108 177 734.8 0 177 109 72 734.8 0 72 110 189 734.8 0 189 111 106 734.8 0 106 112 311 734.8 0 311 113 264 734.8 0 264 114 132 734.8 0 132 115 52 734.8 0 52 116 39 734.8 0 39 117 46 734.8 0 46 118 43 734.8 0 43 119 142 734.8 0 142 120 66 734.8 0 66 121 72 734.8 0 72 122 80 734.8 0 80 123 62 734.8 0 62 124 110 734.8 0 110 125 102 734.8 0 102 126 149 734.8 0 149 127 374 734.8 0 374 128 433 734.8 0 433 129 386 734.8 0 386 130 141 734.8 0 141 131 188 734.8 0 188 132 94 734.8 0 94 133 162 734.8 0 162 134 456 734.8 0 456 135 672 734.8 0 672 136 179 734.8 0 179 137 91 734.8 0 91 138 129 734.8 0 129 139 214 734.8 0 214 140 246 734.8 0 246 141 314 734.8 0 314 142 386 734.8 0 386 143 611 734.8 0 611 144 1085 734.8 0 1085 145 1415 734.8 0 1415 146 30180 734.8 0 30180 147 33177 734.8 0 33177 148 4854 734.8 0 4854 149 368 734.8 0 368 150 280 734.8 0 280 151 525 734.8 0 525 152 1058 734.8 0 1058 153 533 734.8 0 533 154 830 734.8 0 830 155 552 734.8 0 552 156 663 734.8 0 663 157 781 734.8 0 781 158 1101 734.8 0 1101 159 1662 734.8 0 1662 160 2889 734.8 0 2889 161 65634 734.8 0 65634 162 67668 734.8 0 67668 163 7257 734.8 0 7257 164 256 734.8 0 256 165 193 734.8 0 193 166 202 734.8 0 202 167 148 734.8 0 148 168 103 734.8 0 103 169 74 734.8 0 74 170 53 734.8 0 53 171 75 734.8 0 75 172 151 734.8 0 151 173 119 734.8 0 119 174 162 734.8 0 162 175 193 734.8 0 193 176 133 734.8 0 133 177 128 734.8 0 128 178 97 734.8 0 97 179 134 734.8 0 134 180 151 734.8 0 151 181 389 734.8 0 389 182 426 734.8 0 426 183 387 734.8 0 387 184 183 734.8 0 183 185 99 734.8 0 99 186 94 734.8 0 94 187 157 734.8 0 157 188 112 734.8 0 112 189 117 734.8 0 117 190 112 734.8 0 112 191 118 734.8 0 118 192 215 734.8 0 215 193 133 734.8 0 133 194 102 734.8 0 102 195 52 734.8 0 52 196 81 734.8 0 81 197 114 734.8 0 114 198 87 734.8 0 87 199 97 734.8 0 97 200 106 734.8 0 106 201 167 734.8 0 167 202 206 734.8 0 206 203 117 734.8 0 117 204 122 734.8 0 122 205 133 734.8 0 133 206 219 734.8 0 219 207 100 734.8 0 100 208 87 734.8 0 87 209 86 734.8 0 86 210 105 734.8 0 105 211 91 734.8 0 91 212 91 734.8 0 91 213 131 734.8 0 131 214 223 734.8 0 223 215 176 734.8 0 176 216 188 734.8 0 188 217 190 734.8 0 190 218 192 734.8 0 192 219 236 734.8 0 236 220 187 734.8 0 187 221 194 734.8 0 194 222 198 734.8 0 198 223 181 734.8 0 181 224 125 734.8 0 125 225 114 734.8 0 114 226 202 734.8 0 202 227 145 734.8 0 145 228 212 734.8 0 212 229 148 734.8 0 148 230 198 734.8 0 198 231 188 734.8 0 188 232 200 734.8 0 200 233 183 734.8 0 183 234 172 734.8 0 172 235 155 734.8 0 155 236 156 734.8 0 156 237 162 734.8 0 162 238 301 734.8 0 301 239 482 734.8 0 482 240 1297 734.8 0 1297 241 1882 734.8 0 1882 242 949 734.8 0 949 243 367 734.8 0 367 244 345 734.8 0 345 245 169 734.8 0 169 246 150 734.8 0 150 247 98 734.8 0 98 248 53 734.8 0 53 249 92 734.8 0 92 250 121 734.8 0 121 251 249 734.8 0 249 252 292 734.8 0 292 253 199 734.8 0 199 254 294 734.8 0 294 255 246 734.8 0 246 256 160 734.8 0 160 257 142 734.8 0 142 258 141 734.8 0 141 259 105 734.8 0 105 260 122 734.8 0 122 261 179 734.8 0 179 262 154 734.8 0 154 263 164 734.8 0 164 264 157 734.8 0 157 265 206 734.8 0 206 266 166 734.8 0 166 267 565 734.8 0 565 268 572 734.8 0 572 269 488 734.8 0 488 270 589 734.8 0 589 271 380 734.8 0 380 272 285 734.8 0 285 273 150 734.8 0 150 274 262 734.8 0 262 275 275 734.8 0 275 276 364 734.8 0 364 277 768 734.8 0 768 278 1000 734.8 0 1000 279 581 734.8 0 581 280 233 734.8 0 233 281 252 734.8 0 252 282 308 734.8 0 308 283 352 734.8 0 352 284 166 734.8 0 166 285 170 734.8 0 170 286 144 734.8 0 144 287 82 734.8 0 82 288 139 734.8 0 139 289 166 734.8 0 166 290 247 734.8 0 247 291 218 734.8 0 218 292 151 734.8 0 151 293 93 734.8 0 93 294 57 734.8 0 57 295 37 734.8 0 37 296 55 734.8 0 55 297 66 734.8 0 66 298 92 734.8 0 92 299 137 734.8 0 137 300 110 734.8 0 110 301 82 734.8 0 82 Finished in 8.28 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 250,425 Reads with adapters: 1,800 (0.7%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 1,786 (0.7%) Total basepairs processed: 38,166,029 bp Total written (filtered): 485,257 bp (1.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1800 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 10 0.0 3 0 0 0 0 10 18 3 0.0 3 0 0 1 0 2 19 15 0.0 3 0 15 20 1651 0.0 4 1611 37 3 21 6 0.0 4 1 3 1 1 22 1 0.0 4 0 0 1 23 62 0.0 4 61 1 24 1 0.0 4 1 28 5 0.0 4 0 0 0 0 5 29 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 1 100 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 110 2 0.0 4 0 0 0 1 1 111 20 0.0 4 0 0 0 17 3 127 7 0.0 4 0 0 0 0 7 129 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 144 1 0.0 4 1 149 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 171 1 0.0 4 1 218 1 0.0 4 0 0 0 0 1 267 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (34 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 1,786 Reads with adapters: 34 (1.9%) Reads written (passing filters): 1,786 (100.0%) Total basepairs processed: 485,257 bp Total written (filtered): 478,213 bp (98.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 34 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 76.5% C: 5.9% G: 8.8% T: 5.9% none/other: 2.9% Overview of removed sequences length count expect max.err error counts 3 4 27.9 0 4 10 2 0.0 1 0 2 11 1 0.0 1 0 1 256 1 0.0 1 1 257 20 0.0 1 19 1 259 1 0.0 1 1 267 1 0.0 1 1 268 2 0.0 1 2 269 1 0.0 1 1 274 1 0.0 1 1