Control with 10 nM ATTO655-HI + 10 nM unlabelled HI 

93 mikroM ATTO655 HI stock 
100 mikroM HI stock

40 mikroL 100 nM ATTO655 HI 
40 mikroL 100 nM HI 
320 mikroL buffer = 400 mikroL total volume 

10 % lp, 50 ms exposure + 100 ms wt, 4000 frames 
350 mikroL is flushed in to each chamber 

Exp2: chamber 1

Exp 3: chamber 2

Exp 4: chamber 3

Exp 6; chamber 5